OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV)

Authors

SASMAKОV Sobirdjan, Institute of the Chemistry of Plant SubstancesFollow
ASHIRОV Оybek, Institute of the Chemistry of Plant SubstancesFollow
ABDURAKHMANОV Jaloliddin, Institute of the Chemistry of Plant SubstancesFollow
XASANОV Shuhrat, Institute of the Chemistry of Plant SubstancesFollow

DOI

10.51348/M PAU8653

Abstract

The aim of this study is determination of optimal fermentation conditions of the Pichia pastoris yeast expressing the recombinant Pres2-S protein of hepatitis B virus (HBV). For this purpose we investigated the main factors influencing for the cultivation of recombinant strain of the yeast Pichia pastoris pPIC3,5-S-HBsAg - the composition of the nutrient medium, pH and dissolved oxygen (DO). As a result, by enzyme-linked immunosorbent assay (ELISA) the optimal concentration of methanol ≈1.0 %, which is the initiator and the only source of carbon for protein expression in the Mut+ phenotype Pichia pastoris yeast cells was determined. In the selected conditions at the end of the fermentation process, the wet biomass of yeast cells was 420 g/l.

Recommended Citation

Sobirdjan, SASMAKОV; Оybek, ASHIRОV; Jaloliddin, ABDURAKHMANОV; and Shuhrat, XASANОV (2020) "OPTIMAL CONDITIONS OF FERMENTATION OF THE PICHIA PASTORIS YEAST EXPRESSING THE RECOMBINANT PRES2-S PROTEIN (M-HBsAg) OF HEPATITIS B VIRUS (HBV)," CHEMISTRY AND CHEMICAL ENGINEERING: Vol. 2020: No. 3, Article 10.
DOI: 10.51348/M PAU8653
Available at: https://cce.researchcommons.org/journal/vol2020/iss3/10