Development of a method of oriented immobilization of lipases on a solid carrier

Authors

Ziyavitdinov Jamolitdin Fazlitdinovich, Institute of Bioorganic Chemistry, Tashkent, UzbekistanFollow
Inogamov Utkir Kudratillayevich, Institute of Bioorganic Chemistry, Tashkent, UzbekistanFollow
Berdiev Nodir Sharifovich, Institute of Bioorganic Chemistry, Tashkent, UzbekistanFollow
Ishimov Uchkun Jomuradovich, Institute of Bioorganic Chemistry, Tashkent, UzbekistanFollow

Abstract

The methods of oriented immobilization of lipases on a solid carrier - Silochrome C-80 have been developed. The resulting sorbents were tested in the process of obtaining biodiesel from cotton oil. It was shown that immobilized lipase on Silochrome C-80 with NH2 group has the highest transetrification ability and stability, while in sorbents with the enzyme immobilized with -COOH and -SH groups this value was 87 and 53%, respectively. Biocatalysis using an affinity sorbent of immobilized lipase with the –NH2 group has a high operational stability, which, after 240 hours of operation, retains more than 50%, and after 480 hours, 17% retains its initial activity.

Recommended Citation

Jamolitdin Fazlitdinovich, Ziyavitdinov; Utkir Kudratillayevich, Inogamov; Nodir Sharifovich, Berdiev; and Uchkun Jomuradovich, Ishimov (2019) "Development of a method of oriented immobilization of lipases on a solid carrier," CHEMISTRY AND CHEMICAL ENGINEERING: Vol. 2019: No. 3, Article 31.
DOI: https://doi.org/10.70189/1992-9498.1166
Available at: https://cce.researchcommons.org/journal/vol2019/iss3/31