PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS

Authors

Shuhrat Sh. KHASANOV, The Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFollow
Jumanazar Ch MENGTURAYEV, TIIAME National Research University, Tashkent, UzbekistanFollow
Jaloliddin M. ABDURAKHMANOV, Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFollow
Dilmurodjon B. TURDALIYEV, TIIAME National Research University, Tashkent, UzbekistanFollow
Shakhnoz S. AZIMOVA, Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFollow

Abstract

In this study we aimed to obtain a novel recombinant β-galactosidase baculovirus using the Bombyx mori baculovirus – insect cell expression system. A new recombinant plasmid was constructed on the basis of the pBacPAK8 transfer vector and cloned in E. coli cells. Subsequently, a recombinant baculovirus was generated in Bombyx mori cells, and efficient expression of the β-galactosidase gene was observed. The enzymatic activity of the protein was confirmed by the ONPG assay. The results demonstrated that the baculovirus – insect cell system expands the possibilities for producing proteins of industrial significance.

Recommended Citation

KHASANOV, Shuhrat Sh.; MENGTURAYEV, Jumanazar Ch; ABDURAKHMANOV, Jaloliddin M.; TURDALIYEV, Dilmurodjon B.; and AZIMOVA, Shakhnoz S. (2025) "PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS," CHEMISTRY AND CHEMICAL ENGINEERING: Vol. 2025: No. 4, Article 10.
DOI: https://doi.org/10.70189/1992-9498.1691
Available at: https://cce.researchcommons.org/journal/vol2025/iss4/10