Gradient precipitation of recombinant proteins contained in silkworm larvae (Bombyx mori) with ammonium sulphate

Authors

Abdurhakhmanov Jaloliddin, Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFollow
Sasmakov Sobirdjan, Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFollow
Khasanov Shuhrat, Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFollow
Ashirov Оybek, Institute of the Chemistry of Plant Substances, Tashkent, UzbekistanFollow

Abstract

We investigated the protein extract of silkworm larvae. For the first time, an extract containing proteins from silkworm larvae, obtained by fractional precipitation using ammonium sulfate, was studied. As a result of experiment is established, that solutions of ammonium sulfate with a concentration from 20% to 65% are optimal for the sedimentation of protein molecules with a mass in the range of 25 and 45 kDa. Optimal results of the study proposed to use in the process of purification of recombinant proteins.

Recommended Citation

Jaloliddin, Abdurhakhmanov; Sobirdjan, Sasmakov; Shuhrat, Khasanov; and Оybek, Ashirov (2019) "Gradient precipitation of recombinant proteins contained in silkworm larvae (Bombyx mori) with ammonium sulphate," CHEMISTRY AND CHEMICAL ENGINEERING: Vol. 2019: No. 2, Article 16.
DOI: https://doi.org/10.70189/1992-9498.1121
Available at: https://cce.researchcommons.org/journal/vol2019/iss2/16