Study of recombinant protein fractions from the Bombyx mori and Pichia pastoris using "native page" method

Authors

Abdurhakhmanov Jaloliddin, Institute of the Chemistry of Plant SubstancesFollow
Sasmakov Sobirdjan, Institute of the Chemistry of Plant SubstancesFollow
Khasanov Shuhrat, Institute of the Chemistry of Plant SubstancesFollow
Ashirov Оybek, Institute of the Chemistry of Plant SubstancesFollow

Abstract

We have studied the recombinant protein fractions of ̴ 24-34 kDa obtained from Bombyx mori - silkworm larvae and yeast Pichia pastoris. Fractions, containing the recombinant proteins by gel-electrophoresis using the “Native PAGE” method, which allows for differentiation of proteins according to their natural (surface) charge and conformational properties were studied. The results showed that the recombinant protein fraction from Bombyx mori (̴ 34 kDa) consisted of four components and fraction from Pichia pastoris (̴ 24 kDa) consisted one component. The most optimal conditions for the “Native PAGE”- the main gel: Tris HCl (pH-8.8) 244 mM, Bis-Acrylamide 10%, TEMED 0.08%, APS 0.08%; concentration gel: Tris HCl (pH-6.8) 125 mM, Bis-Acrylamide 4%, TEMED 0.05%, APS 0.05% were found.

Recommended Citation

Jaloliddin, Abdurhakhmanov; Sobirdjan, Sasmakov; Shuhrat, Khasanov; and Оybek, Ashirov (2020) "Study of recombinant protein fractions from the Bombyx mori and Pichia pastoris using "native page" method," CHEMISTRY AND CHEMICAL ENGINEERING: Vol. 2020: No. 1, Article 12.
DOI: https://doi.org/10.51348/CHOD7917
Available at: https://cce.researchcommons.org/journal/vol2020/iss1/12